aln-pe.sam

850309 reads, size:604340159 bytes, created 2015-01-07 20:50:37

Mapping stats:

Number of alignments in various mapping quality (MAPQ) intervals and number of unmapped sequences.

Number Percentage
MAPQ >= 30 797672.0 93.8
MAPQ < 30 2641.0 0.3
MAPQ < 20 2786.0 0.3
MAPQ < 10 2090.0 0.2
MAPQ < 3 580.0 0.1
Unmapped 44540.0 5.2
Total 850309.0 100.0

Number of alignments in various mapping quality (MAPQ) intervals and number of unmapped sequences.

Read Length Distributions

  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped

Distribution of read lengths separated by mapping quality thresholds.

Base Quality Distributions

  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped
  • MAPQ >= 30
  • MAPQ < 30
  • MAPQ < 20
  • MAPQ < 10
  • MAPQ < 3
  • Unmapped

Base quality distributions separated by mapping quality thresholds.

Composition of MAPQ >= 20 Reads.

  • A
  • C
  • G
  • T
  • A
  • C
  • G
  • T

A HMM was trained on a subset of the sequences. Shown are log2 odds ratios comparing emission probabilities in match states to background nucleotide probabilities. Values above 0 indicate positional enrichment of a particular nucleotide. "L" indicates the emission probabilities for a state modelling residiues in the middle of the reads.

Composition of 0 >= MAPQ < 20 Reads.

  • A
  • C
  • G
  • T
  • A
  • C
  • G
  • T

A HMM was trained on a subset of the sequences. Shown are log2 odds ratios comparing emission probabilities in match states to background nucleotide probabilities. Values above 0 indicate positional enrichment of a particular nucleotide. "L" indicates the emission probabilities for a state modelling residiues in the middle of the reads.

Composition of unmapped reads.

  • A
  • C
  • G
  • T
  • A
  • C
  • G
  • T

A HMM was trained on a subset of the sequences. Shown are log2 odds ratios comparing emission probabilities in match states to background nucleotide probabilities. Values above 0 indicate positional enrichment of a particular nucleotide. "L" indicates the emission probabilities for a state modelling residiues in the middle of the reads.

Distribution of Mismatches (MAPQ >= 30):

  • A
  • C
  • G
  • T
  • A
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  • G
  • T
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  • T

Distribution of Mismatches in MAPQ >= 30 reads.

Distribution of Mismatches (MAPQ < 30):

  • A
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  • G
  • T
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Distribution of Mismatches in MAPQ < 30 reads.

Distribution of Mismatches (MAPQ < 20):

  • A
  • C
  • G
  • T
  • A
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Distribution of Mismatches in MAPQ < 20 reads.

Distribution of Mismatches (MAPQ < 10):

  • A
  • C
  • G
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Distribution of Mismatches in MAPQ < 10 reads.

Distribution of Mismatches(MAPQ < 3):

  • A
  • C
  • G
  • T
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Distribution of Mismatches in MAPQ < 3 reads.

Number of Errors Per Read (MAPQ >= 30):

  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors

Barplot shows the percentage of reads (y-axis) with 0, 1, 2 ... errors (x axis) for MAPQ >= 30 reads.

Number of Errors Per Read(MAPQ < 30):

  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors

Barplot shows the percentage of reads (y-axis) with 0, 1, 2 ... errors (x axis) for MAPQ < 30 reads.

Number of Errors Per Read(MAPQ < 20):

  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors

Barplot shows the percentage of reads (y-axis) with 0, 1, 2 ... errors (x axis) for MAPQ < 20 reads.

Number of Errors Per Read(MAPQ < 10):

  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors

Barplot shows the percentage of reads (y-axis) with 0, 1, 2 ... errors (x axis) for MAPQ < 10 reads.

Number of Errors Per Read(MAPQ < 3):

  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors
  • Errors

Barplot shows the percentage of reads (y-axis) with 0, 1, 2 ... errors (x axis) for MAPQ < 3 reads.