FastQCFastQC Report
Fri 23 Jan 2015
M120_S2_L001_R1_001.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename M120_S2_L001_R1_001.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 421292
Filtered Sequences 0
Sequence length 35-301
%GC 28

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGAGG 65200 3.4007542 4.2310266 4
CCTCC 62490 3.2364292 3.8956616 8
GGAGC 60100 3.1318362 3.5353942 5
GCTCC 60345 3.1282392 3.5020874 50-59
GGTGG 57855 3.0075226 3.5268037 6
TGGAG 142140 3.0058286 3.430805 4